Detailed view for Tb927.3.3690

Basic information

TDR Targets ID: 16842
Trypanosoma brucei, flagellar radial spoke protein-like, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.1853 | Length (AA): 548 | MW (Da): 60993 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04712   Radial spokehead-like protein

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
97 211 5cwh (A) 7 111 15.00 0 0.4 0.398054 -1.12
205 279 5e4f (B) 355 427 29.00 0.2 0.24 0.338361 -0.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129136)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG3121   CG3121 gene product from transcript CG3121-RA
Echinococcus granulosus EgrG_000210100   radial spoke head protein 4 A
Echinococcus granulosus EgrG_000461300   radial spoke head protein 4 A
Echinococcus multilocularis EmuJ_000210100   radial spoke head protein 4 A
Echinococcus multilocularis EmuJ_000461300   radial spoke head protein 4 A
Giardia lamblia GL50803_16720   Hypothetical protein
Homo sapiens ENSG00000104941   radial spoke head 6 homolog A (Chlamydomonas)
Homo sapiens ENSG00000111834   radial spoke head 4 homolog A (Chlamydomonas)
Leishmania braziliensis LbrM.29.0670   flagellar radial spoke protein-like, putative
Leishmania braziliensis LbrM.13.0250   flagellar radial spoke protein, putative
Leishmania donovani LdBPK_130290.1   Flagellar radial spoke protein 4/6
Leishmania infantum LinJ.29.0690   flagellar radial spoke protein-like, putative
Leishmania infantum LinJ.13.0290   flagellar radial spoke protein, putative
Leishmania major LmjF.29.0660   flagellar radial spoke protein-like, putative
Leishmania major LmjF.13.0430   flagellar radial spoke protein, putative
Leishmania mexicana LmxM.13.0430   flagellar radial spoke protein, putative
Leishmania mexicana LmxM.08_29.0660   flagellar radial spoke protein-like, putative
Mus musculus ENSMUSG00000040866   radial spoke head 6 homolog A (Chlamydomonas)
Mus musculus ENSMUSG00000039552   radial spoke head 4 homolog A (Chlamydomonas)
Neospora caninum NCLIV_033120   GM16080, related
Oryza sativa 4342974   Os07g0298900
Plasmodium berghei PBANKA_0942300   radial spoke head protein, putative
Plasmodium falciparum PF3D7_1104600   radial spoke head protein, putative
Plasmodium knowlesi PKNH_0902200   radial spoke head protein, putative
Plasmodium vivax PVX_090910   hypothetical protein, conserved
Plasmodium yoelii PY02386   Drosophila melanogaster GH13165p
Schistosoma japonicum Sjp_0053540   Radial spokehead-like protein 1, putative
Schistosoma mansoni Smp_134660   radial spokehead-l
Schistosoma mansoni Smp_057090   radial spokehead-l
Schmidtea mediterranea mk4.001966.02   Putative radial spokehead-l
Schmidtea mediterranea mk4.028952.00  
Schmidtea mediterranea mk4.024656.00  
Schmidtea mediterranea mk4.015823.01   Putative radial spokehead-l
Trypanosoma brucei gambiense Tbg972.3.3990   flagellar radial spoke protein-like, putative
Trypanosoma brucei gambiense Tbg972.11.5120   flagellar radial spoke component, putative,Flagellar radial spoke protein 4, putative
Trypanosoma brucei Tb927.11.4480   Flagellar radial spoke protein 4/6
Trypanosoma brucei Tb927.3.3690   flagellar radial spoke protein-like, putative
Trypanosoma congolense TcIL3000.11.4550   Flagellar radial spoke protein 4/6
Trypanosoma congolense TcIL3000_3_2390   flagellar radial spoke protein-like, putative
Trypanosoma cruzi TcCLB.510149.120   flagellar radial spoke protein-like, putative
Trypanosoma cruzi TcCLB.506989.70   flagellar radial spoke protein-like, putative
Trypanosoma cruzi TcCLB.504089.50   Flagellar radial spoke protein 4/6
Toxoplasma gondii TGME49_233330   radial spokehead family protein
Trichomonas vaginalis TVAG_182860   flagellar radial spoke, putative
Trichomonas vaginalis TVAG_455950   flagellar radial spoke, putative

Essentiality

Tb927.3.3690 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.3690 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.3.3690 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.3690 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.3690 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.2060 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.2060 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.2060 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.2060 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0942300 Plasmodium berghei Dispensable plasmo
TGME49_233330 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.3.3690 (Trypanosoma brucei), flagellar radial spoke protein-like, putative
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